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Publications

Peer-Reviewed Publications

Published work and manuscripts in preparation. Authorship position is stated on every entry; work not yet published is labelled as such.

4

Peer-reviewed publications

3

Manuscripts in preparation

15+

Research projects

9

Conference presentations

Published

Corresponding authorQ2 JournalResearch ArticlePublished2026

From Association to Mechanism: Regulatory Annotation and Pathway Mapping of Genes Surrounding Breast Cancer Risk Variants

Sultana Jannat, Trina Mitra, Nafisa Nawar Fariha, Tasmia Tabassum, Kulsuma Bahar Bethi, Synchita Majumder Kaya, Md. Arif Hossen, Md. Saad Hossain, Jobaier Ibne Deen, Mohammed Emon, Laila Khaleda, Md. Hridoy Ahmed

Computational and Systems Oncology, 6, e70024 · Wiley

How this connects. Regulatory annotation of risk variants - the same interpretive step applied to the ADPKD and PCOS cohorts, here on breast cancer loci.

  • Corresponding-author study published open access in Computational and Systems Oncology (Wiley), 2026.
  • Examined 175 breast cancer risk variants confirmed by genome-wide association studies and the genes flanking each locus.
  • Mapped the risk genes onto androgen receptor, Wnt/β-catenin, and Hedgehog signaling pathways central to mammary biology.
  • Annotated correlated variants with HaploReg, finding a 4.8-fold excess of DNase I hypersensitivity and a 15.7-fold excess of enhancer motifs.
First & corresponding authorQ2 JournalReview ArticleIn press2026

Molecular Pharming: Advances, Applications, and Future Prospects in Biotechnology and Medicine

Md. Hridoy Ahmed, Md. Mustak Khan, Shishir Dutta, Md. Foyzur Rahman, Mohammad Shariful Islam, Md. Najmul Hosen, Md. Saad Hossain, Md. Aftabur Rahman, Md. Sadman Hasan Sahil, Tanjuma Tasnim Hira, Ifthesum Ashikur Rahaman, Md. Afser Rabbi, Laila Khaleda

Engineering in Life Sciences · Wiley

How this connects. A review of recombinant protein production in plants, written out of the molecular biology and expression work behind the bench programme.

  • First-author, corresponding-author review accepted in Engineering in Life Sciences (Wiley), February 2026; article page and DOI to follow on final publication.
  • Traces the evolution of molecular pharming and plant-based production of recombinant proteins, vaccines, and industrial enzymes.
  • Surveys host organisms, genetic-engineering methods (including CRISPR/Cas9), and protein extraction and purification strategies.
  • Weighs advantages against challenges of public perception, regulation, and economic sustainability, and maps future directions.
First authorQ1 JournalResearch Article2024

A structure-based drug design approach for the identification of antiviral compounds targeting the Chikungunya virus RdRp protein

Md. Hridoy Ahmed, Gagandeep Singh, Melvin Castrosanto, Alomgir Hossain, Md. Morshedul Islam Rifat, Sadia Hosna Rima, Vandana Gupta, Rajesh K. Kesharwani, Mariusz Jaremko, Abdul-Hamid Emwas, et al.

Chemical Physics Impact, 8, 100450 · Elsevier

How this connects. Where the structural modelling and molecular dynamics methods were built - now applied to asking what disease variants do to protein structure.

  • Designed antiviral candidates through pharmacophore-based virtual screening and molecular docking.
  • Carried out structural analysis, binding free-energy evaluation, and molecular dynamics simulation.
  • Identified multi-target compounds with strong RdRp inhibitory potential.
  • Published in a Q1 Elsevier journal as first author.
First authorQ1 JournalResearch Article2023

An immuno-informatics approach for annotation of hypothetical proteins and multi-epitope vaccine design against the Mpox virus

Md. Hridoy Ahmed, Gagandeep Singh, Melvin Castrosanto, Prawez Alam, Faizul Azam, et al.

Journal of Biomolecular Structure and Dynamics, 42(10), 5288-5307 · Taylor & Francis

How this connects. Proteome-wide screening and epitope prediction - the computational annotation skills that variant interpretation draws on.

  • Identified antigenic proteins through proteome-wide immunoinformatics screening.
  • Designed a multi-epitope vaccine construct targeting Mpox viral proteins.
  • Performed molecular docking with immune receptors, including TLR-4 and MHC alleles.
  • Simulated immune responses using computational immunology tools.

Manuscripts in preparation

Bench-generated data

Three collaborative cohort studies where the DNA was extracted, amplified, and sequenced in our laboratory. None of these are published yet - status is stated on each entry.

In preparationCo-authorClinical Variant Discovery

Variant Spectrum of Autosomal Dominant Polycystic Kidney Disease in a Bangladeshi Cohort: A Nine-Gene Targeted Panel Study

37 patients · 9 controls · 9-gene panel

Methods

  • DNA extraction and targeted panel library preparation
  • Targeted sequencing of PKD1, PKD2, PKHD1 and six further cystic kidney genes
  • GATK HaplotypeCaller with ClinVar and gnomAD v4 annotation
  • Call-set QC by Ti/Tv ratio, mapping quality, and reference-allele verification

Principal finding

Fourteen pathogenic or likely pathogenic variants across PKD1, PKD2 and PKHD1; a candidate variant in 41% of patients. One ClinVar-listed variant was shown to be a mapping artefact. The first ADPKD variant spectrum reported from Bangladesh.

In preparationCo-authorHuman Disease Genetics

Common Polymorphisms and Rare Coding Variants Converge on Gonadotropin, Insulin and Androgen Signalling in Bangladeshi Women with PCOS

300 patients · 300 matched controls

Methods

  • PCR and Sanger genotyping of five FSHR, INSR and SHBG polymorphisms
  • Whole-exome sequencing with GRCh38 alignment and GATK Best Practices
  • Rare-variant prioritisation and protein stability prediction
  • Pathway enrichment analysis

Principal finding

All five polymorphisms associated with PCOS, and rare damaging variants converged on the same reproductive and metabolic pathways - common and rare variation implicating one mechanism.

In preparationCo-authorGenomic Epidemiology & AMR

Clone- and Plasmid-Structured Antimicrobial Resistance in ESBL-Producing Escherichia coli Across Three Regions of Bangladesh

38 isolates · Barishal, Chattogram, Dhaka

Methods

  • Isolate characterisation, DNA extraction, and sequencing library preparation
  • SPAdes assembly, Prokka annotation, Panaroo pan-genome, IQ-TREE phylogeny
  • In silico MLST and Mash comparison against global genomes
  • MOB-suite plasmid reconstruction and mobile-element profiling

Principal finding

Seventeen sequence types resolved: a homogeneous CTX-M-15/ST131 background countrywide, with a focal, Dhaka-centred carbapenemase signal riding international high-risk clones. Resistance is structured by clone and plasmid, not by geography.

Full publication record

For the complete, continuously updated list, see my ORCID and ResearchGate profiles.